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1. Effect Of Diets Formulated With Different Nutrient Densities On Performance And Blood Parameters Of Broilers At Different Phases.

by Hafiz Gulfraz Ahmad (2007-VA-089) | Prof. Dr. TalatNaseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Poultry sector, the second largest industry of Pakistan, is growing exponentially to fulfill the growing needs of meat and eggs. Although poultry sector has shown incredible growth rate during the past decade; however, there is still gap between poultry meat demand and supply. Every living animal has a need for protein. It is the basic structural material from which all body tissues are formed. Dietary protein and metabolize-able energy content has re-emerged as a topic of interest and importance over the last few years mainly due to environmental concerns. Availability of balanced and economical ration has great importance in broiler production. Feed cost is the single most important factor, which accounts more than 70 percent of total cost in poultry production. The challenge for nutritionist and production managers is not only to provide a feed cheaper to a maximum possible level, but also to maximize profitability, because performance of the bird depends upon a broad range of dietary nutrients. Two hundred day old chicks were purchased from the commercial hatchery. On the first day all chicks were weighed and were separated into 4 dietary treatment groups in completely randomized design so that each experimental treatment consist of 5 replicates having 10 chicks in each replicates in this way each treatment consist of total 50 chicks(4x5x10=200). Feeder and waterer were clean before start of experiment and were cleaned regularly till the end of experiment. Feed and water which were provided throughout experiment were clean, Ad libitum and completely hygenized. Four experimental diets were prepared according to the Aviagen Recommendation (2012) but the level of ME in all groups were different, HD feed with ME (3000-3200 Kcal/Kg) and CP (19-24 %), MD feed with ME (2900-3100 Kcal/Kg) and CP (19-23), ND feed with ME (2800- 3000 Kcal/Kg) and CP (19-23 %) and LD feed with ME (2700-2900 Kcal/Kg) and CP (18-23 %) respectively. Data were collected for different parameters of growth performance, carcass traits and blood TG and HDL in mg/dl, respectively. The economics of each treatment was analyzed at the end data was analyzed through Analysis of Variance (ANOVA) with use of Completely Randomized Design. The highest weight gain (1862.4± 106.56) was observed in group HD having (ME: 3000 Kcal/Kg in Starter ration, 3100 Kcal/Kg in grower ration and 3200 Kcal/Kg in finisher ration) and lowest (2974.6±26.96) was observed in group LD having (ME: 2700 Kcal/Kg in Starter ration, 2800 Kcal/Kg in grower ration and 2900 Kcal/Kg in finisher ration) at 35 days of age. Overall result showed that there was significantly decline in weight gain with decreasing level of ME in diet .Statistical analysis showed that all treatments in starter and finisher rations were different (P≤0.05) except in finisher ration. Carcass traits were non-significant (p > 0.05) to each other throughout experiment. Dressing percentage of broilers of different treatment groups was highest (60.38±7.90) in MD group and lowest was observed in LD feed group (54.6±10.37). Dressing percentage was increased as the level of metabolize-able energy increased in diets. The highest blood Triglyceride level (36.3±2.5) was observed in HD group and lowest (29.5±3.92) was observed in ND group. Statistical analysis showed that there was a strong significant difference (P≤0.05) among all these treatment. Same results were found in case of HDL levels in blood. Data analysis proved that MD feed was most economical with good results. Availability: Items available for loan: UVAS Library [Call number: 2308-T] (1).

2. Impact Of Digestible Lysine, Methionine + Cysteine Ratios On Growth Performance, Ileal Digestibility Of Protein And Gut Health In Broilers Fed Different Protein Sources

by Ch. Ahsan Akram (2009-VA-397) | Prof. Dr. Talat Naseer Pasha | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Poultry production is a vibrant segment of agriculture in Pakistan. Genetic selection and nutritional improvements resulted in accelerated growth rate and better FCR in modern broilers. This improved broiler performance requires greater amount of digestible nutrients. Since feeding cost accounts about 70% of total production cost and protein is the most expensive component of poultry diets. Future poultry diets, therefore, will be formulated by using cheaper and poorly digestible protein sources such as CM and SFM due to price volatility of highly digestible but expensive sources like SBM. The undigested protein in hind gut is subjected to microbial fermentation, results in formation of nitrogenous products that are not only harmful to birds, but also excreted into litter bedding and generate ammonia, nitrous acid and nitrous oxide, the latter one is a powerful greenhouse gas with an effect about 300 times compared with carbon dioxide. The potential solution to this problem is supplementation of synthetic amino acids in low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein sources. Therefor a study was designed with the objectives to determine the impact of dLys, M + C ratios on growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). An experiment with 432 mixed sex (Ross 308) 1-d-old broilers was conducted to test the hypothesis that supplementation of Meth may improves growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and dM+C/d Lys (recommended vs. supplemented) were tested in a factorial arrangement of 8 (2 × 2 × 2) dietary treatments. The supplementation of Meth positively influenced FI (P = 0.042), BWG (P = 0.003) and FCR (P <0.001). The broilers fed Meth supplemented diets had, on an average, 10% greater apparent Ileal digestibility of protein, 34% longer villi, 24.5% shorter crypts and 60% greater VCR compared with those fed diets with recommended levels of Meth. The levels of CP, similarly, influenced (P <0.001) the FI, BWG and FCR. The broilers fed high CP diets had 9% improved apparent Ileal digestibility of protein compared with those fed low CP diets. In conclusion, supplementation of Meth improves the broiler growth performance, apparent Ileal digestibility of protein and gut health fed poorly digestible protein sources (CM and SFM). The negative effects of poorly digestible protein sources thus may be partially ameliorated by supplementation of Meth. CONCLUSIONS In conclusion, the negative effects of poorly digestible protein sources thus may be ameliorated by supplementation of Meth. Supplementation of Meth above the recommended levels improves the growth performance, Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). SUGGESTION AND RECOMMENDATION Further research is needed to evaluate the different strategies to improve the digestibility of poorly digestible protein sources including guar meal. Threonine also has an important role in gut health so there is a need to study the effects of supplementation of threonine above the recommended levels in broilers fed poorly digestible protein sources. Feed form has pronounced effects on gut development, digestibility of nutrients and subsequent growth performance of birds. There is a dire need to evaluate the other cheaper protein sources, supplemented with synthetic amino acids and to find out a possible interaction between feed form and poorly digestible protein sources that may replace SBM in broilers. Availability: Items available for loan: UVAS Library [Call number: 2567-T] (1).

3. Effect of Super Doses of Phytase from Two Different Sources on Growth Performance, Phosphorus Utilization and Carcass Characteristics of Broilers

by Faisal Mushtaq (2009-VA-369) | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Cereal grains contain almost two-third of the P that is present in phytate form. Phytate possesses anti-nutritive properties as it binds other nutrients like amino acids and carbohydrates. Birds are unable to use P bound with phytate complex. The phytate P is excreted into the environment causing pollution. To compensate requirements expensive inorganic P sources like DCP and rock phosphate are used in poultry diets, which ultimately increase the ration cost. Exogenous phytase is capable of degrading phytate into lower esters having less chelating capacity. Commercially phytase enzymes are being added in the poultry diets to hydrolyze the phytate P that can replace the expensive inorganic P addition. In the industry, phytase is being added in the poultry diets at 500 FTU/kg. Use of phytase at this rate still leaves some gap. Keeping this thing in view present study was planned to use the super dose of phytase from two different sources (Buttiauxella and E.coli) to minimize the anti-nutritive aspects of phytate and use the phytate P at maximum. For this purpose 35 day feeding trial was conducted to evaluate the effect of super dosing of phytate from two different origins on growth performance, phosphorus utilization and carcass characteristics of broilers. For this in total, 672 one-day old broiler chicks were randomly selected& assigned to 8 dietary treatments, each having 3 replicates of 28 birds. One group was reared on positive control diet formulated according to the Hubbard strain recommended guides with adequate P without any phytase addition. Second group was offered NC diet (deficient in Av.P). While other six diets were formulated with three levels (500, 1000, 1500 FTU/kg) of E.coli and Buttiauxella origin supplemented in NC diet. The FI, BWG and FCR were recorded at the end of each week. At the end of experiment n=3 birds per replicate were selected randomly, weighed and slaughtered to estimate plasma P, ileal digestibility of P, tibia ash, organ weight and dressing percentage. The data collected were analyzed using analysis of variance (ANOVA) SUMMARY 41 technique in a Completely Randomized Design (CRD) and means were be compared through Duncan’s multiple range test (DMR) (Duncan 1955) by using SAS (version 9.1; SAS Inst. Inc., Cary, NC). Results of the experiment revealed that FI, BWG and FCR was higher 48%, 11% and 64.5% respectively in phytase supplemented diet best treatment T8, when compared to the NC. Phytase supplementation had no effect on organ weights. Organ weight was higher in diet low in NPP. Phytase supplementation had no effect on gizzard weight even at higher doses. Similarly phytase supplementation had positive effects on bone mineralization. Phytase addition in the diets improved tibia ash, Ca and P% at all levels (P <0.01). Plasma P level was significantly increased at higher level of phytase. The AIDC of P was linearly increased by phytase supplementation. Considering the economics phytase supplemented diets at higher level 1500 FTU/kg were most economical when cost of feed calculated to attain 1kg of live weight. From this experiment it can be concluded that phytase supplementation at higher dose than the standard ensures the maximum break down of phytate P and reduce its anti-nutritive aspects apart from the source of phytase used in the experiment. Dietary inclusion of phytase at super dose economically generates the bio-available P and reduces the P load in the environment improve growth performance, and bone mineralization. To replace the highly expensive phosphorus sources super dose of phytase can be used instead of standard dose. It can replace the expensive source of inorganic P and economically can generate more profit. Optimum level of phytase in the diet need to be reevaluated to replace inorganic P to some extent, minimize the anti-nutritive aspects of phytate and reduce P load in the environment. Availability: Items available for loan: UVAS Library [Call number: 2607-T] (1).

4. Effect Of Methionine And Mannan Oligosaccharides Supplementation On Growth Performance, Carcass Characteristics, Immunity And Selected Blood Metabolites In Broiler Chicken Under Conditions Of High Ambient Temperature And Humidity

by Asif Mahmood (2009-VA-379) | Dr. Muhammad Afzal Rashid | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Poultry sector is one of the most organized and vibrant segment of agriculture sector in Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200 billion rupees investment and offering employment directly or indirectly to 1.5 million people. Broilers exposed to high environmental temperature and humidity exhibit several physiological and performance related setbacks. Heat stress cause high mortality, poor FCR, reduced feed consumption, body weight gain, and visceral development. Heat stress has been linked to a decrease in intestinal immune activity and to impaired intestinal morphology following pathogenic bacteria invasion of the body through the intestinal epithelium. Nutritional manipulations with its low cost are preferred to alleviate the negative effects of heat stress on performance. Nutritional strategies such as the addition of prebiotics, some amino acids e.g. methionine are reported to be beneficial in alleviating heat stress. As a nutritional intervention, synthetic methionine added to the diet has been explored as method of ameliorating the effects of ROS associated with high environmental temperature. Keeping above in view, the present study has been planned to evaluate the effect of supplementing methionine and MOS on the growth performance, carcass characteristics and immune status against ND under field conditions. In this experiment a total of n=200 day old chicks were procured from a commercial hatchery and randomly assigned to dietary treatments with n=40 chicks per treatment. Each treatment was further divided into four replicates with n=10 birds/replicate. Dietary treatments were : 1) negative control (NC) without any supplementation, 2) positive control (PC) containing antibiotic, 3) MOS supplemented (MOS) containing 800 and 400 g/ton of MOS in the starter and grower, respectively, 4) High Methionine supplemented treatment (HM) containing Methionine 1% of the diet in both starter and grower feeds, 5) Methionine and MOS supplemented (HM+MOS) treatment containing high methionine SUMMARY 42 (1% in starter and grower) along with 800 and 400 g/ton of MOS in the starter and grower, respectively. The temperature and humidity were recorded daily at 8:00, 14:00 and 18:00 h and means were calculated to determine the daily changes in temperature humidity index. Feed intake, body weight and FCR were recorded at the end of each week. At the end of experiment n=3 birds per replicate were selected, weighed and slaughtered. At the time of slaughtering blood samples were collected in evacuated tubes. Serum was harvested after centrifugation, dived into aliquots and stored at –20 oC until further analysis. Serum samples were used to determine antibody titer against ND. At the time of slaughtering organ weighed and jejunal sample were collected to determine the gut morphology. The data collected were analyzed using analysis of variance (ANOVA) technique in a Completely Randomized Design (CRD). Results were declared statistically significant at P < 0.05 and compared using Tukey, s multiple comparison test. The statistical analysis were carried out using SAS 9.1 (SAS Institute, 2006). Live BW was higher (P<0.05) in HM+MOS compared to NC and HM. Feed intake was higher (P<0.05) in HM+MOS and PC than those in NC. However, FCR was lowest in HM+MOS compared to NC and PC birds. Relative organ weights were similar (P>0.05) among treatments; however, bursa of fabricius was heavier in MOS compared to the NC and HM+MOS. Villus height and villus to crypt ratio was highest (P<0.05) in HM, than the NC treatment birds CONCLUSIONS In conclusion, supplementation of methionine at 1% of diet and MOS improved the weight gain, feed intake and feed conversion ratio in birds reared under conditions of high temperature and humidity. Gut morphology of the bird also improved by supplementation of Methionine and MOS in the broiler diet. SUMMARY 43 SUGGESTION AND RECOMMENDATION Further research is needed to re-determine the recommended levels of methionine in the diet when bird are reared under the conditions of high temperature and humidity. Availability: Items available for loan: UVAS Library [Call number: 2606-T] (1).

5. Protease Supplementation Influences Growth Performance, Gut Health And Ileal Digestibility Of Protein In Broilers Fed Low Protein Diets

by Shahzad Ahmad (2008-VA-240) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Proteases are protein-digesting enzymes that are used to break down the proteins of various plant materials and proteinaceous anti-nutrients in plant protein feedstuffs. The use of exogenous proteases can help in the reduction of the protein contents of a given feed by improving the digestibility and availability of the protein thereby reducing the cost of feed contributed by the protein feedstuff. It will also help to reduce the nitrogen excretion by the poultry and will contribute to mitigate the climate change. In the present study the protease from a bacillus lachniformis spp was supplemented to evaluate the effect of enzyme on protein digestibility, gut health and birds performance. For this three experimental diets with 21, 19 and 17 % CP (HP, MP and LP) with six treatments with and without exogenous protease were used in this trial to evaluate the effect of protease. Each treatment was replicated to 6 pens and 10 birds were allocated to each pen. Parameters studied regarding birds performance were feed intake, weight gain and FCR. All the growth performance parameters were recorded on weekly basis while the Ileal digestibility and gut health parameters were recorded at the 35th day of the trial. Data was analysed through completely randomized design (CRD) under factorial arrangement using ANOVA technique (Steel et al. 1997). Means were separated through Duncan’s Multiple Range test (Duncon’s, 1955) with the help of SAS 9.1. The results of feed intake, weight gain and feed intake: gain on weekly basis showed inconsistent results, however the overall performance of the birds fed low protein diets supplemented with protease showed significant results. The gut health parameters showed the significant increase in villus height. The apparent Ileal digestibility of protein was significantly increased in the MP supplemented with protease enzyme. 36 CONCLUSION Based upon the findings of this study it can be concluded that;  Supplementation of protease improves growth performance of the broilers however the results on weekly basis were inconsistent but overall growth performance data shows significant results.  Protease supplementation enhances the CP digestibility in 19 % diets compared to 21 % and 17 % CP diets.  The supplementation of protease enzyme improves the villus height and crypt depth: villus height in broilers. SUGGESTIONS AND RECOMMENDATIONS  Future researchers can further evaluate the subsequent effects of the protease on growth performance and immune response in broilers.  In future the research can be conducted to determine the effect of protease on energy digestibility and immune response in broilers. Availability: Items available for loan: UVAS Library [Call number: 2601-T] (1).

6. Effect Of Post Pelleting Liquid Phytase Supplementation On Growth Performance And Phosphorous Utilization In Broilers

by Muhammad Balal Munawar (2014-VA-516) | Prof. Dr. Saima | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Poultry sector is one of the most developed and vibrant segment of agriculture sector in Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200 billion rupees investment and offering employment directly or indirectly to 1.5 million people. In vegetable sources used in poultry rations, P is mostly present in phytate form. Phytate P is not digested by poultry due to lack of endogenous phytase enzyme. So exogenous phytase are now added in feed to release P from phytate. Phytase is mainly derived from fungi (Aspergilus niger) and bacteria (Escherichi coli). Phytase starts work either at 3rd C (3-Phytase) or at 6thC (6-Phytase) of phytate Phytase mostly used in pre pelleting powder form but it has some activity issues during high pelleting temperature. Steam application is the most important factor that reduces enzyme activity due high steam temperature and addition of moisture ultimately lead to enzyme inactivity. Post pelleting liquid enzyme can be one solution to combat enzyme activity issue. In total, 504 1-d-old chicks were procured from a commercial hatchery and randomly assigned to six dietary treatments with Hubbard straight run n=84 chicks per treatment. Each treatment was further divided into 3 replicates with 28 birds/replicate. One treatment was served as positive control, while second was served as negative control with (0.30 and 0.26 %) P in starter and grower respectively. Other four diets had two levels (500 and 750 FTU/kg) of each powder and liquid enzyme. Liquid enzyme supplementation was done post pelleting. Pelleting temperature was kept at 85o C. Feed intake, body weight gain and FCR was recorded at the end of each week. At 35th day of experiment, 3 birds per replicate were selected randomly, weighed and slaughtered. At the time of slaughtering, blood samples were collected in heparinized tubes for the determination of plasma SUMMARY 40 P.Ileal digesta from the slaughtered birds was collected for the determination of P digestibility. The visceral organs and left tibia from the slaughtered birds were collected, organ weight was recorded and tibias were ashed for determination of Calcium and P. Data collected was analyzed using analysis of variance (ANOVA) technique under Completely Randomized Design (CRD) and means were compared through DMR test by using SAS 9.1 version (SAS 2001). The conducted study showed 49.45%, 64.64%, and 8.64 % higher results in feed intake, body weight gain and FCR when best group H compared with group A (NC). Liver and heart weight increase in group A, however gizzard weight showed non significant (p>0.05) results among all groups. Tibia ash, Phosphorous, calcium and plasma P showed significant results (p<0.05) when all phytase supplemented diets compared with NC. Availability: Items available for loan: UVAS Library [Call number: 2631-T] (1).

7. Effects Of Digestible Lysine Levels On Production Efficiency, Protein Digestibility, Gut Health And Carcass Characteristics In Broilers Fed Different Protein Sources

by Muhammad Jhazaib Arslan (2014-VA-956) | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: In Pakistan, poultry is a vibrant part of agriculture. Nutritional and genetic improvements resulted in accelerated PEF in broilers. This improved broiler production efficiency requires greater amount of digestible nutrients like digestible amino acids. Feeding cost accounts for 70% of total cost and protein is the most expensive component of broilers ration. Future poultry rations will be formulated by using poorly digestible and cheaper protein sources such as SFM and CM due to price instability of highly digestible sources like SBM. The undigested protein in hind gut subjected to microbial fermentation, results in formation of nitrogenous products that are harmful to birds. The possible solution to this problem is supplementation of synthetic amino acids in low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein sources. Therefore a study was designed with the objectives to determine the impact of dLys on production efficiency, TTP digestibility, gut health and carcass characteristics in broilers fed poorly digestible protein sources (CM and SFM). An experiment with 624 Ross 308 1-d-old broilers was conducted to test the hypothesis that supplementation of dLys may improve production efficiency, TTP digestibility, gut health and carcass characteristics of the broilers fed poorly digestible protein sources (CM and SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and dLys (high, medium and low) were tested in a factorial arrangement of 12 (2 × 2 × 3) dietary treatments and 1 control treatment. The supplementation of dLys positively influenced production efficiency (P <0.001). The birds fed diets containing 19% CP showed 8.53% greater TTP digestibility compared with those fed diets with 17% CP, however, VH was influenced by protein source (P<0.001) and CP level (P = 0.030), however, not influenced by dLys levels while CD was influenced by dLys levels (P = 0.023). The birds fed 1.16% dLys supplemented diet had a 9.6 and 7.5% greater dressed weight with giblets compared with those birds fed diets with 1.10 and 1.21% level of dLys. In conclusion, supplementation of dLys improves the broiler production efficiency fed poorly digestible protein sources (CM and SFM). The negative effects of poorly digestible protein sources thus may be partially ameliorated by supplementation of dLys. CONCLUSIONS Supplementation of dLys above the recommended levels (1.16%) improves the production efficiency, TTP digestibility of protein, gut health and carcass characteristics in broilers fed poorly digestible protein sources (CM and SFM). In conclusion, the efficiency of poorly digestible protein sources may be improved by supplementation of dLys at level of 1.16%. Availability: Items available for loan: UVAS Library [Call number: 2765-T] (1).

8. Influence Of Digestible Lysine Levels On Growth Performance, Gut Development And Ileal Digestibility Of Two Vegetable Protein Sources Fed To Broiler Birds

by Anjum Faiz (2014-VA-955) | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: The poultry sector is playing a substantial role in agriculture sector of Pakistan. The extensive research in poultry has brought vital changes that accelerated growth and improved FCR. Poultry diets are majorly formulated cost effective for the sustainable poultry industry. For this reason, the main focus is on protein sources which, if maintained to an adequate level with the requirement results in cost effective feeding. In the coming days, main focus of the nutritionists would be on replacement of expensive sources of protein during feed formulation. For this reason, their first choice may be the selection of poorly digestible protein sources such as CM and SFM. These sources are cheaper as compare to the SBM which is imported hence increasing the feed cost. The poorly digestible protein sources, furthermore, in hind gut may be subjected to microbial fermentation, results in formation of nitrogenous products that are not only harmful to birds, but also excreted into litter bedding and generate ammonia, nitrous acid and nitrous oxide, the latter one is a powerful greenhouse gas with an effect about 300 times compared with carbon dioxide. The potential solution to this problem is supplementation of synthetic amino acids in low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein sources. For this reason, a study was designed with the objectives to determine the impact of dLys, on growth performance, ileal digestibility of protein and gut development in broilers fed poorly digestible protein sources (CM and SFM). An experiment with 624 mixed sex 1-d-old broilers (Ross 308) was conducted to test the hypothesis that supplementation of dLys may improves growth performance of the broilers fed poorly digestible protein sources (CM and SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and dLys (recommended vs. supplemented) were tested in a factorial arrangement of 12 (2 × 2 × 3) dietary treatments and SBM as a control diet was used. The results indicate the birds fed diets DISCUSSION 42 containing CM and SFM supplemented with dLys has significant effect on growth performance in broilers. The results of the study showed that the FI, BWG and FCR for CM and SFM based diets was different during starter and grower phases. The FI and BWG FCR was influenced by CM and SFM based diets. The FI was not influenced by dLys levels during starter phase. The BWG and FCR was improved with supplementation of dLys levels. During starter phase FI and BWG was not influenced by CP levels, however, FCR was improved. The results of the grower phase indicate that FI was significantly influenced by protein sources, CP levels, as well as, by dLys levels. The BWG, similarly, was significantly influenced by protein sources, CP levels, and dLys levels. The FCR during the grower phase was improved by increasing CP level. The FCR was also improved when fed CM based diets. Supplementation of dLys significantly influenced FI and BGW during grower phase. The FCR was also improved with the supplementation of dLys levels. The results indicate that the birds fed dLys supplemented diets had significant effect on ileal digestibility of protein. The results of the study showed that ileal digestibility for protein was not influenced by CP levels. The present study resulted that some of the organs are significantly influenced by dLys levels and CP levels, whereas, other are in influenced by protein sources. Amino acid supplementation showed significant difference on ileal digestibility of protein and gut development in broilers. The negative effect of poorly digestible lysine can be compensated with the supplementation of synthetic amino acids including lysine. CONCLUSIONS Supplementation of dLys above the recommended levels improves the growth performance, ileal digestibility of protein and gut development in broilers fed poorly digestible protein sources (CM and SFM). In conclusion, the negative effects of poorly digestible protein sources in broilers may be ameliorated by supplementation of dLys. Availability: Items available for loan: UVAS Library [Call number: 2776-T] (1).

9. Effect Of Feed Form And Particle Size Of Corn On Growth Performance, Protein Digestibility And Gut Health Of Broiler

by Shahzada Muhammad Zeshan Haider (2014-VA-919) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: The presented study was conducted at control house of Department of Poultry Production, C-block, UVAS Ravi Campus. In total 480, day-old Hubbard Classic broiler chicks were procured from local hatchery, weighed at 1st day and randomly distributed to eight treatment groups in such a way that there were six replicates in each group and each replicate had 10 chicks. Eight isocaloric and isonitrogenous experimental diets were formulated. Half birds were offered crumble form of feed in whole life while half birds were offered crumble form in starter phase and pellet form in finisher phase. Four levels (<1, 1-1.5, 1.5-2 and 2-2.5mm) of corn particle size were used in each form of feed.Body weight and feed consumptionwere recorded weekly in order to calculate feed conversion ratio.Data collected were analyzed by ANOVA using factorial arrangement under CRD. Experimental means were compared using DMR test. Birds fed diet having higher particle size (2-2.5mm) of corn had lower feed consumption, greater body weight gain and improved FCR compared to those fed smaller particle size during starter, finisher and overall phase. Further, birds fed pelleted feed had lower feed consumption, greater weight gain and improved FCR than those fed crumble form of feed.Further, different particle size of corn resulted in non-significant effect on villus height and crypt depth. Feed form also had non-significant influence on villus height and crypt depth of duodenum.Birds fed diet having higher particle size (2-2.5mm) of corn had higher apparent ileal digestibilityof protein than those fed smaller particle size. Feed form had non-significant effect on apparent ileal digestibilityof protein. It can be concluded from the results that birds fed pelleted diet containing coarse particle size had improved growth performance and protein digestibility without adverse effect on gut health of broiler. Availability: Items available for loan: UVAS Library [Call number: 2879-T] (1).



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